TY - JOUR T1 - Peptide YY Inhibits Intestinal Cl<sup>−</sup> Secretion in Experimental Porcine Cryptosporidiosis Through a Prostaglandin-Activated Neural Pathway JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 692 LP - 697 VL - 283 IS - 2 AU - Robert A. Argenzio AU - Martha Armstrong AU - Anthony Blikslager AU - J. Marc Rhoads Y1 - 1997/11/01 UR - http://jpet.aspetjournals.org/content/283/2/692.abstract N2 - Peptide YY (PYY) is a powerful inhibitor of intestinal secretion mediated by cAMP agonists such as vasoactive intestinal peptide and prostaglandin E2. We hypothesized that PYY would attenuate the secretory diarrhea in piglet cryptosporidiosis, which is mediated by prostaglandins E2 and I2. Control and infected ileal tissues from piglets were studied in Ussing chambers. The addition of PYY to the serosal bathing solution abolished net Cl− secretion in infected tissue. The inhibitory effect of PYY was eliminated with the prostaglandin synthesis inhibitor indomethacin and with the nerve conduction blocker tetrodotoxin. PYY completely blocked the antiabsorptive and secretory effects of the prostaglandin I2 analog carbacyclin, which has previously been shown to operate through enteric nerve pathways in this tissue. In contrast, PYY had no inhibitory effect on the secretory responses induced by prostaglandin E2 or vasoactive intestinal peptide. Results suggest that the antisecretory effects of PYY are mediated by inhibition of prostaglandin I2 induction of enteric nerves. Thus, PYY may play an important role in moderating the secretory diarrhea in cryptosporidiosis. The American Society for Pharmacology and Experimental Therapeutics ER -