PT  - JOURNAL ARTICLE
AU  - Susan F. Law
AU  - Terry Reisine
TI  - Changes in the Association of G Protein Subunits with the Cloned Mouse <em>Delta</em> Opioid Receptor on Agonist Stimulation
DP  - 1997 Jun 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 1476--1486
VI  - 281
IP  - 3
4099  - http://jpet.aspetjournals.org/content/281/3/1476.short
4100  - http://jpet.aspetjournals.org/content/281/3/1476.full
SO  - J Pharmacol Exp Ther1997 Jun 01; 281
AB  - G proteins couple delta opioid receptors to multiple cellular effector systems and are critical components of thedelta opioid signal transduction cascade. To investigate the physical association of delta opioid receptors with G proteins, the cloned mouse delta opioid receptor was solubilized, and the G proteins associated with the receptor were identified through coimmunoprecipitation of the receptor/G protein complexes with antisera directed against different Gα and Gβ subunits. The delta receptor associates with Giα1, Giα3, Goα, Gβ1 and Gβ2 subtypes. On agonist binding to the receptor, a greater proportion of the receptor is associated with Giα than with Goα, Giα1dissociates from the receptor and Giα2 associates with the receptor, whereas Giα3 and the Gβsubunits remain coupled to the delta receptor. These findings reveal dynamic changes in the G proteins associated with the receptor after agonist binding that may be linked to the activation of the delta receptor. In addition to pertussis toxin-sensitive G proteins, the delta receptor physically interacts with the pertussis toxn-insensitive G proteins Gqα and Gzα. These interactions may be critical in linkingdelta receptors to phospholipase C. The diversity of G proteins associated with the delta opioid receptor may form the basis for the selective coupling of these receptors to multiple cellular effector systems. The American Society for Pharmacology and Experimental Therapeutics