RT Journal Article
SR Electronic
T1 Acetylcholine-induced chloride current oscillations in swine tracheal smooth muscle cells.
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 178
OP 186
VO 276
IS 1
A1 X Liu
A1 J M Farley
YR 1996
UL http://jpet.aspetjournals.org/content/276/1/178.abstract
AB The activation of muscarinic receptors by acetylcholine (ACh) in tracheal smooth muscle cells induced Ca++ oscillations, measured as the activation of Ca(++)-dependent Cl- currents (Clca) at the K+ equilibrium potential. The currents were not abolished by replacement of external and internal K+ with Cs+ but decreased after reduction in internal Cl- concentration or extracellular application of niflumic acid, a Cl- channel blocker. The Clca oscillations were dependent on external Ca++ concentration ([Ca++]e). The mean current and frequency increased with increasing [Ca++]e, were enhanced by Bay K 8644 and were inhibited by verapamil, suggesting a role for voltage-operated Ca++ channels (VOC). Steady-state increases in Clca induced by 10(-6) M ACh could be converted to oscillations by reducing [Ca++]e or by buffering intracellular Ca++ with ethyleneglycolbis-N,N,N'-N'-tetraacetic acid (EGTA). Oscillations in Clca induced by 3 x 10(-8) and 10(-7) M ACh were more sensitive to EGTA than those induced by 10(-6) M ACh. Caffeine induced nonoscillatory, transient increases in Clca and reduced subsequent ACh-induced increases in Clca. The oscillatory patterns of Clca induced by ACh and the effects of modification of Ca++ influx were similar for oscillations in intracellular Ca++ concentration [Ca++]i as measured with confocal microfluorimetry. Thus, ACh-induced Clca oscillations reflect fluctuations in [Ca++]i that are consistent with initiation of Ca++ release from inositol-1,4,5-trisphosphate-(IP3)sensitive Ca++ stores. Maintenance of the oscillations requires Ca++ influx, in part through voltage-operated Ca++ channels.