PT  - JOURNAL ARTICLE
AU  - A Botella
AU  - M Delvaux
AU  - J Fioramonti
AU  - J Frexinos
AU  - L Bueno
TI  - Receptor subtypes involved in dual effects induced by prostaglandin E2 in circular smooth muscle from dog colon.
DP  - 1995 Jun 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 1008--1014
VI  - 273
IP  - 3
4099  - http://jpet.aspetjournals.org/content/273/3/1008.short
4100  - http://jpet.aspetjournals.org/content/273/3/1008.full
SO  - J Pharmacol Exp Ther1995 Jun 01; 273
AB  - Smooth muscle strips and isolated muscle cells from the circular layer of dog colon, were used to study the effect of prostaglandin E2 (PGE2) and their analogs acting at EP receptors: Iloprost (IP/EP1), butaprost (EP2) and enprostil (EP3) and SC19220 (antagonist EP1) to characterize the EP-receptors involved in the control of muscle function. In strips treated with tetrodotoxin, only enprostil provoked a concentration-dependent contraction. The concentration of enprostil inducing a half maximal contraction (EC50) was 400 nM and the maximal effect was obtained at 1 microM. PGE2, butaprost and iloprost induced a dose-dependent relaxation, with an EC50 of 200, 80 and 200 nM, respectively. The maximal relaxation was obtained at 1 microM for all these agents. When the EP1 antagonist, SC19220 (10 microM), was added 20 min before PGE2 or their analogs, their respective concentration-response curves were not affected. In isolated cells, PGE2 and enprostil induced a cell contraction in a concentration-dependent manner, whereas iloprost and butaprost had no effect by themselves. The maximal contraction was 241.1 +/- 1.7% at 10 nM PGE2 and 22.5 +/- 1.6% at 10 nM enprostil. EC50 of PGE2 and enprostil was 40 pM. SC 19220, at concentrations ranging from 1 pM to 0.1 microM, failed to inhibit the contraction induced by either PGE2 or enprostil. When cells were preincubated for 1 min with butaprost or iloprost at concentrations ranging from 1 pM to 1 microM, the contraction induced by CCK8 (10nM) was inhibited in a concentration-dependent matter.(ABSTRACT TRUNCATED AT 250 WORDS)