RT Journal Article
SR Electronic
T1 In vivo renal production and tubular secretion of tyramine.
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 1187
OP 1192
VO 272
IS 3
A1 J W Van Huysse
A1 D P Henry
A1 L R Willis
YR 1995
UL http://jpet.aspetjournals.org/content/272/3/1187.abstract
AB Para-tyramine (p-TYM) is a predominant urinary amine in humans, rabbit, rat and dog, and its urinary excretion rate may reflect central nervous system pathophysiology. However, the source of urinary p-TYM is not known, nor have the mechanisms regulating its excretion been characterized. The present study, by using renal clearance techniques, examined the sources of urinary p-TYM and the mechanism of excretion in anesthetized rabbits. In all studies, the renal clearance of p-TYM was compared with that of norepinephrine (NE). Base-line delivery to the kidney of p-TYM in plasma was 8.6 +/- 1.6 ng/min (mean +/- S.E.M., n = 16), whereas the mean urinary excretion rate of p-TYM was 26.5 +/- 3.6 ng/min during the same period (P < .001 vs. delivery). In three separate series of experiments, either vehicle (n = 5) or a specific inhibitor of renal tubular organic cation secretion, cyanine 863 (6 mg/kg, n = 7), or a specific inhibitor of aromatic-amino acid-decarboxylase, alpha-mono-fluoromethyldopa (FMD, 4 mg/kg, n = 5), were infused i.v., Mean arterial pressure, glomerular filtration rate, renal plasma flow and urine flow rate were unchanged in all studies. The renal clearances of p-TYM (Cp-TYM) and NE (CNE) were unchanged only after vehicle. After cyanine 863, Cp-TYM was decreased to 36% of control (P < .01), whereas CNE decreased to 21% of its base-line value (P < .01). After FMD Cp-TYM was reduced to 2% of control (P < .05), whereas CNE decreased by 44% (P < .01).(ABSTRACT TRUNCATED AT 250 WORDS)