PT  - JOURNAL ARTICLE
AU  - M A Mahajan
AU  - M Acara
AU  - M Taub
TI  - Uptake and phosphorylation of thiamine in rabbit primary proximal tubule cells and Madin Darby canine kidney cells. II. Effect of ethanol.
DP  - 1994 Mar 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 1316--1320
VI  - 268
IP  - 3
4099  - http://jpet.aspetjournals.org/content/268/3/1316.short
4100  - http://jpet.aspetjournals.org/content/268/3/1316.full
SO  - J Pharmacol Exp Ther1994 Mar 01; 268
AB  - Uptake of [14C]thiamine was studied in renal primary proximal tubule cells and in Madin Darby Canine Kidney cells in culture. Findings were compared with data for the accumulation of [14C]thiamine and its phosphorylation in renal cortical slices. There was a saturable component for thiamine uptake in all cell types. When normalized for milligrams of protein, renal cortical slices accumulated 82% less 14C than renal primary proximal tubule cells and 51% less than distal tubule cells. Maximal [14C]thiamine levels accumulated by the saturable component was 1.33 nmol/g in slices and 7.02 nmol/g in proximal tubule cells. Ethanol, at 25 mM, inhibited thiamine uptake and phosphorylation in the cell cultures similar to its effect in the kidney tissue.