RT Journal Article
SR Electronic
T1 Specific binding sites for leukotriene B4 in guinea pig brain membranes.
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 1035
OP 1042
VO 259
IS 3
A1 A J de Brum-Fernandes
A1 G Guillemette
A1 P Borgeat
A1 P Sirois
YR 1991
UL http://jpet.aspetjournals.org/content/259/3/1035.abstract
AB The presence of specific leukotriene B4 (LTB4) binding sites was investigated in membranes prepared from mouse, rat, guinea pig and rabbit brain. Specific binding sites have been found in guinea pig brain membranes (GPBM) but not in the other species studied. Specific binding of LTB4 to GPBM was proportional to membrane concentration, saturable and reversible, reaching a steady-state suggesting equilibrium after 30 min of incubation. Analysis of the binding data showed a single binding site with a Kd = 2.27 +/- 0.55 nM and a Bmax = 576 +/- 89.6 fmol/mg protein. The relative potencies of LTB4 analogs to displace the tritiated ligand were LTB4 greater than 20-hydroxy-LTB4 much greater than 20-carboxy-LTB4 greater than 12-(S)hydroxy-5,8,10,14(Z,Z,E,Z)-eicosatetraenoic acid; LTD4 and LTC4 did not displace the ligand in concentrations up to 50 microM. The binding was inhibited by monovalent cations and GTP[gamma S] and increased by divalent cations. Specific binding was associated to the cerebral cortex. The present results suggest that these specific binding sites could represent functional LTB4 receptors in the guinea pig cerebral cortex with unknown biological effects.