TY - JOUR T1 - Diurnal variation of the rat vas deferens contraction induced by stimulation of presynaptic nicotinic receptors and pineal function. JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 614 LP - 619 VL - 259 IS - 2 AU - R C Carneiro AU - J Cipolla-Neto AU - R P Markus Y1 - 1991/11/01 UR - http://jpet.aspetjournals.org/content/259/2/614.abstract N2 - A rhythmic variation of maximal contraction induced by acetylcholine in the prostatic portion of rat vas deferens was tested. This contraction is due to the release of norepinephrine and ATP from sympathetic nerve terminals. Male Wistar rats (4 months old) were housed on a light/dark cycle (12 hr/12 hr, lights on at 6:00 A.M.). The diurnal variation of acetylcholine-induced contraction was determined on animals sacrificed every 3 hr during the day. The maximal contractile response shows a circadian (24:00 hr) and an ultradian (12:20 hr) rhythm. Otherwise, the sensitivity to acetylcholine (pD2 values) and the maximal contraction or pD2 values to norepinephrine, ATP and K+ did not change throughout the day. The blocking effect of hexamethonium on the contraction induced by field stimulation was higher at 9:00 P.M. than at 3:00 P.M., indicating a diurnal variation of the effect of endogenous released acetylcholine. When melatonin released by the pineal gland is suppressed by constant illumination or superior cervical ganglionectomy, the circadian rhythm was abolished and the period of the ultradian rhythm changed to 6:30 hr. The acetylcholine-induced contraction of vasa deferentia from animals sacrificed at 3:00 P.M. and incubated "in vitro" with melatonin (100 pg/ml) increased reaching nocturnal values. In conclusion, it seems that a functional pineal gland, most probably through the synthesis and release of melatonin, is necessary for expression (circadian) and modulation (ultradian) of the rhythmicity in the maximal acetylcholine-induced contraction in the prostatic portion of the rat vas deferens. ER -