RT Journal Article
SR Electronic
T1 Transport of cyclosporin A in kidney epithelial cell line (LLC-PK1).
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 200
OP 204
VO 257
IS 1
A1 A Takayama
A1 Y Okazaki
A1 K Fukuda
A1 M Takano
A1 K Inui
A1 R Hori
YR 1991
UL http://jpet.aspetjournals.org/content/257/1/200.abstract
AB The characteristics of cyclosporin A transport have been studied in cultured kidney epithelial cell line LLC-PK1. The uptake of cyclosporin A by LLC-PK1 cells was time-dependent and reached a steady state at about 30 min. The initial uptake was saturable and was inhibited by cyclosporin A analogs, cyclosporin C and D and by verapamil, but not by metabolic inhibitors such as 2,4-dinitrophenol and rotenone. Cyclosporin A uptake as well as efflux was strongly dependent on temperature. The Arrhenius plot for cyclosporin A uptake was biphasic, whereas the Arrhenius plot for sulfanilamide uptake, which is transported by a simple diffusion, was linear. These results indicate that a specific mechanism is concerned in the transport of cyclosporin A in LLC-PK1, cells.