PT  - JOURNAL ARTICLE
AU  - A G King
AU  - K S Landreth
AU  - D Wierda
TI  - Bone marrow stromal cell regulation of B-lymphopoiesis. II. Mechanisms of hydroquinone inhibition of pre-B cell maturation.
DP  - 1989 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 582--590
VI  - 250
IP  - 2
4099  - http://jpet.aspetjournals.org/content/250/2/582.short
4100  - http://jpet.aspetjournals.org/content/250/2/582.full
SO  - J Pharmacol Exp Ther1989 Aug 01; 250
AB  - Previous investigations from our laboratory have shown that the benzene metabolite, hydroquinone (HQ), inhibits B cell production by preventing the maturation of pre-B cells. Data presented in this paper demonstrate that HQ interrupts B-lymphopoiesis indirectly by inhibiting the production of interleukin-4 (IL-4) by fibroblastic stromal cells. HQ exposure of fibroblastic stromal cells (SCL-173 and SCL-160) did not affect IL-4 production by these cell lines at any dose tested. Addition of untreated bone marrow-derived macrophages to HQ-treated bone marrow stromal cells (heterogeneous population of fibroblastic cells and macrophages) reversed inhibition of IL-4 production. However, addition of HQ-treated macrophages was without effect. Our studies suggest that HQ inhibits macrophage production of IL-1, a potent inducer of IL-4 production by bone marrow fibroblastic stromal cells. Interruption of IL-1 release from macrophages mediates the observed inhibition of B lineage cell maturation.