TY - JOUR T1 - Kindled seizures result in decreased responsiveness of benzodiazepine receptors to gamma-aminobutyric acid (GABA). JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 147 LP - 150 VL - 226 IS - 1 AU - R J Fanelli AU - J O McNamara Y1 - 1983/07/01 UR - http://jpet.aspetjournals.org/content/226/1/147.abstract N2 - Repeated kindled seizures result in increased numbers of benzodiazepine receptors in fascia dentata membranes. Our previous work localized these receptors to a discrete neuronal population, the dentate granule cells. The present investigation characterizes the regulation of this binding by NaCl, pentobarbital and gamma-aminobutyric acid (GABA). [3H]Flunitrazepam binding in membranes from kindled rats exceeded that from controls by 24%. NaCl increased this binding in membranes prepared from control and kindled animals by roughly equivalent amounts (18 and 14%, respectively). No significant differences were found in the effects of pentobarbital on benzodiazepine binding between kindled and control groups. In contrast, addition of GABA increased benzodiazepine binding by a significantly smaller percentage in the kindled (116 +/- 5%) compared to the control (138 +/- 11%) group. We propose that this altered molecular response to GABA is intimately related to the enhanced GABA-mediated inhibition of granule cells present after kindled seizures. ER -