PT - JOURNAL ARTICLE AU - E Widerlöv AU - C D Kilts AU - R B Mailman AU - C B Nemeroff AU - T J Mc Cown AU - A J Prange, Jr AU - G R Breese TI - Increase in dopamine metabolites in rat brain by neurotensin. DP - 1982 Oct 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 1--6 VI - 223 IP - 1 4099 - http://jpet.aspetjournals.org/content/223/1/1.short 4100 - http://jpet.aspetjournals.org/content/223/1/1.full SO - J Pharmacol Exp Ther1982 Oct 01; 223 AB - Neurotensin (NT), an endogenous tridecapeptide, is heterogeneously distributed in the central nervous system. The present study examined the effects of physiologically and behaviorally active doses of NT (1--100 micrograms intracisternally) on dopamine, serotonin and their primary metabolites as well as accumulation of dopa after inhibition of dopa decarboxylase. NT was shown to increase dopa accumulation when compared with saline treatment, suggesting that dopamine synthesis was increased. In accord with this view, NT also caused a dose-dependent increase in homovanillic acid and dihydroxyphenylacetic acid, the major metabolites of dopamine, in several brain areas (striatum, olfactory tubercles, nucleus accumbens, frontal cortex and hypothalamus). Interestingly, the increase in homovanillic acid was greater than that for dihydroxyphenylacetic acid. In striatum, an initial increase in dopamine content after 30 micrograms of NT was followed by an increase and a subsequent decrease of dopamine metabolites. Several other neuropeptides (Met-enkephalin, cholecystokinin-8, thyrotropin releasing hormone, substance P and d-Arg9-NT), at doses equimolar to 30 micrograms of NT, did not affect dopamine metabolites, whereas certain others (beta-endorphin and bombesin) increased their concentration in some brain areas. Except for the highest dose of NT, measures of serotonergic function were not affected by NT or any of the other neuropeptides.