@article {Quest255, author = {J A Quest and G S Rowles and H B Bhat and R A Gillis}, title = {Effect of dihydro-ouabain on vascular tone on the perfused cannine hindlimb.}, volume = {199}, number = {1}, pages = {255--261}, year = {1976}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Digitalis compounds are known to cause contraction of vascular smooth muscle. However, it has been reported by Kahn and colleagues (J. Pharmacol. Exp. Ther. 142: 215-222, 1963) that dihydro-ouabain has no effect on vascular tone in dogs. The purpose of our study was to reassess the vasoconstrictor activity of dihydro-ouabain. This was done by monitoring perfusion pressure of the canine hindlimb preparation perfused at a constant rate of blood flow and administering intra-arterial injections of dihydro-ouabain. Increasing doses of this drug (i.e., 25, 50, 100, 200 and 300 mug) produced significant dose-dependent increases in perfusion pressure. Pretreatment with phentolamine prevented the effect of dihydro-ouabain on perfusion pressure. These results indicate that dihydro-ouabain causes contraction of vascular smooth muscle by an adrenergic mechanism.}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/199/1/255}, eprint = {https://jpet.aspetjournals.org/content/199/1/255.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }