RT Journal Article
SR Electronic
T1 An enzymatic derivative double isotope assay for measuring tissue levels of taurine.
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 301
OP 308
VO 193
IS 1
A1 J B Lombardini
YR 1975
UL http://jpet.aspetjournals.org/content/193/1/301.abstract
AB The development of a new assay for taurine is described. The procedure utilizes the formation of taurocholic acid by rat liver microsomes and is dependent upon the dilution of the specific activity of radioactive taurine by the amount of taurine present in perchloric acid homogenates of tissues. Known amounts of taurine (21/2-300 nmol) are added to incubation vessels to establish a standard curve so that unknown quantities of taurine present in tissue homogenates can be calculated by a graphical comparison. The possible interference by other compounds present in tissue extracts and their removal by passage through columns containing both cation and anion exchange resins is discussed. Conditions are established for the quantitative recovery of taurine after passage through the exchange resins. Measurement of the taurine levels of several rat tissues is presented along with duplicate assays performed on the amino acid analyzer for comparison purposes.