@article {Feldman358, author = {Harvey A. Feldman and Irwin Singer}, title = {COMPARATIVE EFFECTS OF TETRACYCLINES ON WATER FLOW ACROSS TOAD URINARY BLADDERS}, volume = {190}, number = {2}, pages = {358--364}, year = {1974}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Demeclocycline causes nephrogenic diabetes insipidus in man and inhibits antidiuretic hormone (ADH)-induced water flow (W) in toad urinary bladders. To clarify the possible mechanism for this action, the effects of five serosally applied tetracycline derivatives on ADH- and cyclic adenosine 3{\textquoteright}, 5{\textquoteright}-monophosphate (cAMP)-induced W were studied in paired toad urinary hemibladders (Bufo marinus). Inhibition of cAMP-induced W was not clearly dose-related and did not correlate with any known physicochemical property of the tetracyclines. However, the relative inhibition of ADH-induced W by these drugs was dose-dependent, and followed the sequence: demeclocycline \> doxycychine \> minocycline \> tetracycline \> oxytetracycline. This sequence correlates exactly with the relative degree of serum protein binding of these drugs, but not with divalent cation chelation or lipid solubility, suggesting that ADH inhibition requires binding to cell membrane proteins important in ADH-induced water transport. {\textcopyright} 1974 by The Williams \& Wilkins Co.}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/190/2/358}, eprint = {https://jpet.aspetjournals.org/content/190/2/358.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }