TY - JOUR T1 - EFFECTS OF VERATRUM ALKALOIDS ON MEMBRANE POTENTIAL AND CONDUCTANCE OF SQUID AND CRAYFISH GIANT AXONS JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 143 LP - 154 VL - 184 IS - 1 AU - MASAHIRO OHTA AU - TOSHIO NARAHASHI AU - RICHARD F. KEELER Y1 - 1973/01/01 UR - http://jpet.aspetjournals.org/content/184/1/143.abstract N2 - Eleven alkaloids obtained from Veratrum have been compared for their effects on the membrane potential and conductances of squid and crayfish giant axons. They can be classed in three groups. 1) Veratridine, cevadine and protoveratrines A and B cause the membrane to depolarize. The potency decreases in that order, and the concentrations of veratridine and cevadine required for 50% maximum depolarization are estimated to be 3.3 x 10-5 M and 3.7 x 10-3 M, respectively. The depolarization by veratridine is due primarily to a selective increase in resting sodium permeability of time membrane and is antagonized by tetrodotoxin. All of them are effective in augmenting and prolonging the negative (depolarizing) afterpotential. 2) Veratramine, isorubijervine, muldaimine (5-veratranine-3β, 11α-diol-11-acetate) and 5-veratranine-3β, 1lα-diol (1 x 10-4 M) are capable of blocking the action potential with little or no depolarization. 5-Veratranine-3β, 1lα-diol blocks both sodium and potassium conductance increases. 3) Cyclopamine, jervine, rubijervine and veratrosine have no effect on the resting and action potentials. Possible structure-activity relationships for these effects are discussed. © 1973 by The Williams & Wilkins Company ER -