RT Journal Article
SR Electronic
T1 QUANTITATIVE DETERMINATION OF MORPHINE IN SERUM BY RADIOIMMUNOASSAY
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 253
OP 258
VO 178
IS 2
A1 SPECTOR, SYDNEY
YR 1971
UL http://jpet.aspetjournals.org/content/178/2/253.abstract
AB The haptenic group morphine was coupled to a protein and the conjugate possessed immunogenic properties. The method for preparing the carboxymethylmorphine protein conjugate is described. Evidence is presented for the formation of antibodies with specificity for the alkaloid as demonstrated by precipitin reaction and by binding of dihydromorphine-8H by the antisera. The details of a simple and convenient procedure for the immunoassay of morphine in plasma are described in this report. The absolute sensitivity of the radioimmunoassay in serum was 50 to 100 pg of morphine in a final assay mixture of 0.5 ml. Levels of morphine in serum from four rats given morphine sulfate (5 mg/ kg i.v.) showed a biphasic decay curve. The first phase had a T1/2 of approximately 50 minutes, whereas the second phase had a T1/2 of 6.8 hours. © 1971 by The Williams & Wilkins Co.