RT Journal Article
SR Electronic
T1 DAUNORUBICIN METABOLISM BY RAT TISSUE PREPARATIONS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 567
OP 572
VO 177
IS 3
A1 NICHOLAS R. BACHUR
A1 MALCOLM GEE
YR 1971
UL http://jpet.aspetjournals.org/content/177/3/567.abstract
AB The antibiotic, daunorubicin (Dl), is metabolized extensively by homogenates of several rat tissues to the fluorescent metabolites, daunorubicinol (D2), daunorubicin aglycone (D4) and daunorubicnol aglycone (D3). The conversion of D1 to D2 is carried out by the 105,000 x g supernatant solution of all tissues examined, is stimulated by reduced triphosphopyridine nucleotide and does not require oxygen. Since the metabolism of Dl → D2 is seen in all tissues tested, including blood cells, it is proposed that a constitutive soluble enzyme, probably a reductase, is responsible for this transformation. The aglycones, D3 and D4 are formed from D2 and Dl, respectively, by liver microsomal preparations with reduced triphosphopyridine nucleotide as a co-factor, but this activity is also significant in homogenates of brain and heart. This activity is presumably a hydrolase. The data indicate the major pathway for daunorubicin metabolism is [See pdf for figure] in tissue homogenates. © 1971 by The Williams & Wilkins Co.