RT Journal Article
SR Electronic
T1 THE MECHANISMS BY WHICH ETHACRYNIC ACID AFFECTS ION CONTENT, ION FLUXES, VOLUME AND ENERGY SUPPLY IN THE RAT UTERUS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 563
OP 579
VO 176
IS 3
A1 E. E. DANIEL
A1 A. M. KIDWAI
A1 KATHLEEN ROBINSON
A1 D. FREEMAN
A1 SYLVIA FAIR
YR 1971
UL http://jpet.aspetjournals.org/content/176/3/563.abstract
AB In rat uterine tissues ethacrynic acid (10-3 M) caused gain of Na and 1120 and loss of K, after two to three hours incubation. Adenosine triphosphate (ATP) levels were also markedly decreased at this time (10-5 and 10-4 M ethacrynic acid had no effect after 6 hours). A decrease in Na efflux, an increase in K efflux and possibly an increase in Na influx began at the same time, whereas a decrease in K influx began earlier. The decrease in ATP and adenosine diphosphate content produced by ethacrynic acid was shown to involve complete inhibition of ATP supply from oxidative phosphorylation and partial inhibition of ATP supply from glycolysis. Ethacrynic acid could also inhibit Na+ + K+-activated and other adenosine triphosphatases of uterine microsomes. The insensitivity of the tissue to ethacrynic acid was not a consequence of its exclusion from the cells, which concentrated ethacrynic acid. Most of the tissue ethacrynic acid was tightly bound and could not be removed by washing. It remained bound to tissue components after homogenization It was concluded that ethacrynic acid affected ion movements in uteri mainly by inhibiting the liberation of energy, and that it acted on both oxidative and glycolytic sources. © 1971 by The Williams & Wilkins Co.