RT Journal Article
SR Electronic
T1 FURTHER EVALUATION OF AN <em>INTRA VITAM</em> STAINING METHOD FOR THE DETECTION OF EARLY <em>IN VIVO</em> ANTISCHISTOSOMAL EFFECTS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 455
OP 463
VO 176
IS 2
A1 JOHN G. BOURGEOIS
A1 ERNEST BUEDING
YR 1971
UL http://jpet.aspetjournals.org/content/176/2/455.abstract
AB One of the earliest effects of a number of structurally unrelated antischistosomal drugs is damage to the female reproductive organs. This is demonstrable by the use of an intra vitam staining method with a tetrazolium salt. The administration of potassium antimony tartrate, niridazole, nicarbazin and p-rosaniline to mice infected with mature Schistosoma mansoni resulted in detectable damage to the reproductive organs of female worms within 5 minutes, 4 to 6 hours, 12 hours and 3 days, respectively. Although the effects produced by each of these four drugs were reversed in the five months after treatment, no such reversal was seen after this time interval when the mice were treated with sublethal doses of a nitrofuran derivative. Although these changes can be brought about also by compounds which selectively produce only a reversible functional damage to the female reproductive system of the worm, it is suggested that this procedure could he useful for the rapid detection of compounds which have potential chemotherapeutic activity in experimental schistosomiasis. © 1971 by The Williams &amp; Wilkins Co.