RT Journal Article
SR Electronic
T1 INHIBITION OF LIPOLYSIS IN ISOLATED FAT CELLS BY SULFHYDRYL REAGENTS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 135
OP 140
VO 171
IS 1
A1 DEANE N. CALVERT
A1 JOHN J. LECH
YR 1970
UL http://jpet.aspetjournals.org/content/171/1/135.abstract
AB The sulfhydryl reagents iodoacetic acid, iodoacetamide, p-chloromercuribenzoate and N-ethylmaleimide were capable of inhibiting norepinephrine-stimulated lipolysis in isolated adipose cells. In addition, iodoacetamide inhibited lipolysis stimulated by theophylline and by dibutyryl cyclic adenosine monophosphate. The data indicate a probable intracellular site of action, possibly upon a triglyceride lipase. The dithiol dimercaprol was capable of preventing the inhibitory action of the compounds but could not reverse established inhibition. The monothiol mercaptoethanol could prevent the inhibition of N-ethylmaleimide. Elucidation of the ultimate mechanism of inhibition by these compounds awaits isolation of the entire system for lipase activation from adipose tissue. © 1970, by The Williams & Wilkins Company