RT Journal Article
SR Electronic
T1 INHIBITORY EFFECTS OF ALLOXAN ON MAMMALIAN ADENYL CYCLASE
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 80
OP 86
VO 169
IS 1
A1 K. L. COHEN
A1 M. W. BITENSKY
YR 1969
UL http://jpet.aspetjournals.org/content/169/1/80.abstract
AB The reported antagonism by caffeine of the diabetogenic effect of alloxan prompted examination of a possible action of alloxan on adenyl cyclase. The effects of alloxan on rat liven, heart, kidney and brain and hamster islet cell adenoma adenyl cyclase were studied in in vitro. An assay based on chromatographic isolation of C14-cycic 3', 5'-adenosine monophosphate was used. Alloxan was found to inhibit adenyl cyclase selectively, reversibly and, in part, competitively. Alloxan, in a concentration of 10-3 M, produced about 80% inhibition in all tissues examined. In view of the role of adenyl cyclase in the regulation of insulin secretion, it is felt that the inhibition of this en zyme by alloxan might be related to the molecular mechanism of action of this drug in vivo. © 1969, by The Williams & Wilkins Company