RT Journal Article
SR Electronic
T1 STIMULATION OF CEREBROSPINAL FLUID PRODUCTION BY LOW DOSES OF INTRAVENTRICULAR OUABAIN
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 581
OP 585
VO 154
IS 3
A1 W. W. Oppelt
A1 R. F. Palmer
YR 1966
UL http://jpet.aspetjournals.org/content/154/3/581.abstract
AB Cerebrospinal fluid (CSF) production was measured by ventriculocisternal perfusion with inulin containing CSF-like buffer in anesthetized cats using low concentrations of intraventricular ouabain, 10-9 M to 10-14 M. Definite stimulation of CSF production, averaging about 45%, was noted at 1O-10 M, with no significant changes at the other concentrations. Na-K adenosine triphosphatase was assayed in cat choroid plexus in vitro, using standard methods and varying ouabain concentrations. Stimulation of Na-K-activated activity was noted between 10-9 and 10-12 M ouabain; however, the results were not entirely consistent. It is concluded that CSF production in the cat is another example of a system where one can observe a biphasic effect of varying doses of ouabain: inhibition of flow and choroid plexus enzyme activity at high doses, and stimulation of both modalities at low, "pharmacologic" doses. © 1966 by The Williams & Wilkins Co.