PT  - JOURNAL ARTICLE
AU  - Bernard Dubnick
AU  - D. Fay Morgan
AU  - Carol A. Towne
AU  - George E. Phillips
TI  - FLUOROMETRIC ASSAY OF QUINDONIUM BROMIDE AND IDENTIFICATION OF A GLUCURONIDE AS THE MAJOR METABOLITE
DP  - 1966 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 301--306
VI  - 153
IP  - 2
4099  - http://jpet.aspetjournals.org/content/153/2/301.short
4100  - http://jpet.aspetjournals.org/content/153/2/301.full
SO  - J Pharmacol Exp Ther1966 Aug 01; 153
AB  - An assay procedure for quindonium bromide (W3366A, an octahydro-8-hydroxy-benzo-cyclopenta-quinolizinium bromide) bsed on its pH-dependent fluorescence is described. Tissues are homogenized in pH 10 borate to prevent breakdown of quindonium glucuronide and are extracted at that pH with butanol-1. The quindonium is reextracted from the butanol-1 into a small volume of 0.1 N HCl. After adjustment of the aqueous extract back to pH 10, fluorescence at 420 mµ is measured upon activation at 360 mµ. The identification of a biotransformation product, which is the major metabolite appearing in the urine, as a monoglucuronide was based on its hydrolysis catalyzed by β-glucuronidase, its incorporation of C14 from radioactive glucose, the purification by column chromatography of a material containing conjugated quindonium and glucuronic acid in a molar ratio of 1:1 and the reaction of quindonium with uridine-5'-diphospho-glucosidouronate catalyzed by microsomes. The Williams & Wilkins Comapny