RT Journal Article
SR Electronic
T1 A CELLULAR ACTION OF MERCURIAL DIURETICS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 23
OP 31
VO 147
IS 1
A1 Jones, Vernon D.
A1 Lockett, George
A1 Landon, Erwin J.
YR 1965
UL http://jpet.aspetjournals.org/content/147/1/23.abstract
AB Diuretic and nondiuretic organic mercurials, added in vitro, inhibit the (Na+ and K+)-activated component of the ATPase system of membrane fragments prepared from rat kidneys. These compounds also reduce the ability of the membrane preparation to stimulate cytoplasmic glycolysis. The (Na+ and K+)-activated ATPase activity was reduced when membrane fragments were prepared from kidneys of rats pretreated with therapeutic doses of diuretic mercurials. The ability of these membrane preparations to stimulate cytoplasmic glycolysis was also decreased. Pretreatment with nondiuretic mercurials at equivalent levels did not result in a decreased ability of membrane preparations to stimulate glycolysis. The ATPase activity of the membrane fragments was not significantly inhibited until the mercurial dose was 8 mg Hg/kg. Thus, a membrane enzyme system that can be correlated with active Na+-K+ transport has been shown to be inhibited by mercurial diuretics in vitro and in vivo. The Williams & Wilkins Comapny