RT Journal Article
SR Electronic
T1 METABOLISM OF CARBON TETRACHLORIDE AND CHLOROFORM BY THE RAT
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 141
OP 148
VO 141
IS 2
A1 Benoy B. Paul
A1 David Rubinstein
YR 1963
UL http://jpet.aspetjournals.org/content/141/2/141.abstract
AB Eighteen hours after intraduodenal administration of C14Cl4, 85% had been excreted in the expired air unaltered and 1% had been converted to C14O2. After administration of C14HCl3, 70% had appeared in the expired air unchanged and 4% as C14O2. Liver slices also formed CO2 more rapidly from CHCl3 than from CCl4. The metabolism of CCl4 and CHCl3 is greatly diminished if the tissues are homogenized. The production of CO2 from chloromethanes by liver slices is stimulated by citrate and acetate, inhibited by iodoacetate, fluoride arsenate, cyanide and by acid, alkali or heat treatment of the liver slices. Succinate stimulated the metabolism only of CCl4, not of CHCl3. Neither reduced glutathione nor cysteine increases CO2 production from CCl4. Some CCl4 is converted to CHCl3in vivo and in vitro. No evidence of the formation of other chloromethanes from either CHCl3 or CCl4 was obtained. It is concluded that CCl4 and CHCl3 are converted to CO2 by enzyme-catalyzed reactions and that CHCl3 probably is an intermediate in the metabolism of CCl4.