RT Journal Article
SR Electronic
T1 A FLUORIMETRIC METHOD FOR THE DETERMINATION OF A MAJOR SPIRONOLACTONE (ALDACTONE) METABOLITE IN HUMAN PLASMA
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 312
OP 316
VO 135
IS 3
A1 Gochman, Nathan
A1 Gantt, Clarence L.
YR 1962
UL http://jpet.aspetjournals.org/content/135/3/312.abstract
AB A fluorimetric procedure, involving simple extraction techniques, has been described for the determination of 0.005 µg or more of a major metabolite appearing in plasma after oral administration of spironolactone. Data obtained by fluorescence characteristics and paper chromatography indicate that the metabolite is the de-thioacetylated derivative, 3-(3-oxo-17β-hydroxy-4,6-androstadien-17α-yl) propionic acid γ-lactone. Plasma levels of this metabolite versus time curves are shown for human subjects after oral administration of spironolactone.