@article {Burack25, author = {W. R. Burack and P. R. Drask{\'o}czy and N. Weiner}, title = {ADENINE NUCLEOTIDE, CATECHOLAMINE AND PROTEIN CONTENTS OF WHOLE ADRENAL GLANDS AND HEAVY GRANULES OF RESERPINE-TREATED FOWL}, volume = {133}, number = {1}, pages = {25--33}, year = {1961}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The disproportionately greater loss of catecholamines than adenine nucleotides from fowl adrenal glands which is known to occur during severe (80 to 95\%) depletion after reserpine administration has been observed during moderate (55 to 65\%) depletion. The fall in equivalence ratio catecholamines/adenine nucleotides after reserpine pretreatment has been measured in extracts both of whole glands and of heavy granules. The percent of whole gland catecholamines recovered in the heavy granule fractions from glands of 15 fowl pretreated with a depleting dose of reserpine (10 mg i.v.) 24 to 72 hours before death (31.1 {\textpm} 3.4 S.E.) was as high as in those from glands of 18 controls (30.4 {\textpm} 1.7 S.E.). Therefore, neither increased mechanical fragility nor lysis of heavy granules was associated with reserpine-induced depletion. The mean concentration catecholamines/mg protein in the heavy granule fractions of the depleted glands was 6 x that in the {\textquoteleft}mitochondrial{\textquoteright} fraction, and since the same relation held for the control glands it is concluded that the degree of homogeneity of the heavy granule fraction of the 2 groups was comparable. In heavy granules of depleted glands the mean concentration of catecholamines was 0.49 and in heavy granules of controls 1.36 {\textmu}mol/mg protein. In the depleted heavy granules catecholamine concentration was closely correlated with that in whole gland (r = 0.83) and inspection of the extrapolated regression line yields information compatible with the view that there is a small extragranular distribution space for catecholamines. Heavy granule protein was found to constitute as much as 35\% of total protein in fowl adrenal glands and therefore an even larger proportion of the medulla protein. The protein content of depleted heavy granule fractions (121 {\textpm} 6.2 mg/g wet wt) was not different from that of control heavy granules (123 {\textpm} 4.6).}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/133/1/25}, eprint = {https://jpet.aspetjournals.org/content/133/1/25.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }