TY - JOUR T1 - TRITIUM LABELED CHLORAMPHENICOL IN <em>E. COLI</em> JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 1 LP - 5 VL - 132 IS - 1 AU - H. Felsenfeld AU - C. E. Carter Y1 - 1961/04/01 UR - http://jpet.aspetjournals.org/content/132/1/1.abstract N2 - Tritiated chloramphenicol which had entered E. coli cells was not associated with the soluble RNA fraction. A soluble fraction was prepared from 33,500 x g ribonucleoprotein particles which actively incorporated labeled amino acids in vitro. This fraction, and the residual 33,500 x g insoluble particle, exhibited a higher ratio of radioactivity to protein than the 105,000 x g supernatant fraction when prepared from cells exposed to tritiated chloramphenicol. However, an exchange reaction between H3-chloramphenicol and protein upon acid precipitation restricted interpretation of this observation. ER -