TY - JOUR T1 - SUBCELLULAR LOCALIZATION OF RADIOACTIVE DIGITOXIN JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 115 LP - 119 VL - 124 IS - 2 AU - James L. Spratt AU - George T. Okita Y1 - 1958/10/01 UR - http://jpet.aspetjournals.org/content/124/2/115.abstract N2 - The essential experimental findings of this study are listed as follows. Twenty minutes after a single intravenous injection, the rat essentially clears its vascular system of digitoxin with a significant amount of metabolism of the parent compound. Upon cell fractionation of in vivo preparations, over 90% of the unchanged digitoxin present in the cell is found in the soluble supernate of rat heart, kidney and liver cells. No marked quantitative binding could be demonstrated with any of the particulate fractions. The results of the in vitro experiments agree with the in vivo work. Washing of the particulate fractions does lower the binding capacities of these fractions, but does not change the gross distribution picture. The presence or absence of nonradioactive drug in the suspending media exerts no significant effect on the binding distribution. ER -