PT  - JOURNAL ARTICLE
AU  - Hofstee, B. H. J.
TI  - A HOMOLOGOUS SERIES OF SOLUBLE n-FATTY ACID ESTERS (C<sub>2</sub>-C<sub>10</sub>) AS SUBSTRATES FOR SERUM CHOLINESTERASE
DP  - 1958 Jun 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 108--113
VI  - 123
IP  - 2
4099  - http://jpet.aspetjournals.org/content/123/2/108.short
4100  - http://jpet.aspetjournals.org/content/123/2/108.full
SO  - J Pharmacol Exp Ther1958 Jun 01; 123
AB  - The activity of "cholinesterase" in human blood serum with the sodium salts of a homologous series of n-fatty acid esters of m-hydroxybenzoic acid as the substrates increases in a characteristic manner with increasing acyl carbon chain length up to at least 10 C atoms. Depending on the substrate and its concentration, the enzymic hydrolysis of these anionic esters is increased up to the order of 20- to 30-fold by certain bivalent metal ions, notably Mg++, Ca++, Sr++, Ba++ and Mn++, but these ions are not essential for activity. The results provide an improvement of a direct and continuous spectrophotometric method for the assay of serum cholinesterase presented previously.