PT  - JOURNAL ARTICLE
AU  - James A. Pittman
AU  - Benton B. Westfall
TI  - SPECTROPHOTOMETRIC ESTIMATION OF AMPHENONE IN BIOLOGIC MATERIAL AND ITS ABSORPTION AND EXCRETION IN MAN
DP  - 1957 Jan 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 64--68
VI  - 119
IP  - 1
4099  - http://jpet.aspetjournals.org/content/119/1/64.short
4100  - http://jpet.aspetjournals.org/content/119/1/64.full
SO  - J Pharmacol Exp Ther1957 Jan 01; 119
AB  - Amphenone concentration in biological material can be determined by diazotization using the Bratton-Marshall reagent to form a magenta dye-complex, which can then be measured spectrophotometrically at 560 λ. Absorption of amphenone from the gastrointestinal tract is rapid, the peak concentration after a single oral dose being reached within one to one and one-half hours in patients with normal renal function. A significant concentration remains after 12 hours, allowing accumulation on the usual dosage regimen. In blood it is distributed throughout plasma and red cells, and is approximately sixty per cent bound in the plasma. The major portion is rapidly excreted via the kidneys. Some may be excreted via the gastrointestinal tract, into which it is secreted. The rate of excretion is diminished when renal function is impaired.