PT  - JOURNAL ARTICLE
AU  - Chao Zhang
AU  - Wenfeng Zeng
AU  - Yi Yao
AU  - Bin Xu
AU  - Xiuli Wei
AU  - Luoyang Wang
AU  - Xiaozhe Yin
AU  - Apurba Kumar Barman
AU  - Fayun Zhang
AU  - Chunling Zhang
AU  - Qibin Song
AU  - Wei Liang
TI  - Naringenin ameliorates radiation-induced lung injury by lowering IL-1β level
AID  - 10.1124/jpet.118.248807
DP  - 2018 Jan 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - jpet.118.248807
4099  - http://jpet.aspetjournals.org/content/early/2018/06/04/jpet.118.248807.short
4100  - http://jpet.aspetjournals.org/content/early/2018/06/04/jpet.118.248807.full
AB  - Purpose: Radiation-induced lung injury (RILI) is the main complication of radiotherapy for thoracic malignancies. Naringenin as a potent immune-modulator has been found to relieve bleomycin-induced lung fibrosis by restoring the balance of disordered cytokines. Thus, we ought to determine whether naringenin would mitigate RILI and investigate the underlying mechanism. Methods: Animals received fractioned irradiation in thoracic area to induce RILI, ELISA and Milliplex were used for serum and bronchoalveolar lavage fluid (BALF) cytokines analysis, haematoxylin & eosin staining for pathological changes and masson trichrome staining for fibrosis determination in the lungs. Results: IL-1β was found significantly elevated after thoracic irradiation and it triggered production of pro-fibrotic TGF-β both in vivo and in vitro, suggesting the vital role of IL-1β in the development of RILI. Furthermore, we found that naringenin was able to ameliorate RILI through down-regulating IL-1β and maintaining the homeostasis of inflammatory factors. Conclusions: Our results demonstrated that naringenin could serve as a potent immune-modulator to ameliorate RILI, more importantly, a new complementary strategy by maintaining the inflammatory factors homeostasis combined with radiation could improve the efficacy of thoracic radiotherapy.