PT  - JOURNAL ARTICLE
AU  - Priscila Hess Lopes
AU  - Marisa M.T. Rocha
AU  - Alexandre Kazuo Kuniyoshi
AU  - Fernanda Calheta Vieira Portaro
AU  - Luís Roberto C. Gonçalves
TI  - Edema and Nociception Induced by <em>Philodryas patagoniensis</em> Venom in Mice: A Pharmacological Evaluation with Implications for the Accident Treatment
AID  - 10.1124/jpet.116.239640
DP  - 2017 Jun 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 349--354
VI  - 361
IP  - 3
4099  - http://jpet.aspetjournals.org/content/361/3/349.short
4100  - http://jpet.aspetjournals.org/content/361/3/349.full
SO  - J Pharmacol Exp Ther2017 Jun 01; 361
AB  - We have investigated the mechanisms involved in the genesis of edema and nociception induced by Philodryas patagoniensis venom (PpV) injected into the footpad of mice. PpV induced dose-related edema and nociceptive effects. Pretreatment of mice with cyclooxygenase inhibitor (indomethacin), but not with cyclooxygenase 2 inhibitor (celecoxib) markedly inhibited both effects. Pretreatments with H1 receptor antagonist (promethazine) or with dual histamine-serotonin inhibitor (cyproheptadine) failed in inhibiting both effects. In groups pretreated with captopril (angiotensin-converting enzyme inhibitor) the edema was unaltered, but nociception was clearly increased, suggesting the participation of kinins in the pathophysiology of the nociception but not of the edema-forming effect of PpV. When PpV was treated with EDTA, the nociception was similar to the one induced by untreated venom, but edema was markedly reduced. We concluded that PpV-induced edema and nociception have cyclooxygenase eicosanoids as the main mediators and no participation of vasoactive amines. Kinins seem to participate in nociception but not in edema induced by PpV. The results also suggest that metalloproteinases are the main compounds responsible for the edema, but not for the nociception induced by this venom.