Abstract
The bisdioxopiperazines, including dexrazoxane (ICRF-187), are catalytic or noncleavable complex-forming inhibitors of DNA topoisomerase II that do not produce DNA strand breaks. In this study we show that dexrazoxane inhibits the division of Chinese hamster ovary (CHO) cells resulting in marked increases in cell size (up to 80 μm in diameter), volume (up to 150-fold greater), and ploidy (as high as 32N). This last result indicates that the dexrazoxane-induced DNA reduplication was restricted to once per cell cycle. Kinetic analysis of the flow cytometry data indicated that the conversion between successively higher ploidy levels was progressively slowed at longer times of exposure to dexrazoxane. Both the protein and DNA content of dexrazoxane-treated CHO cells increased linearly over time in the same proportion. Light and electron microscopic studies of dexrazoxane-treated cells showed ring-like multilobulated nuclei. Immunohistochemical staining of dexrazoxane-treated cells showed that F-actin and acetylated α-tubulin were present in large, highly organized networks. Immunohistochemical staining of the dexrazoxane-treated CHO cells also showed that the topoisomerase IIα colocalized with the DNA of the multilobulated nuclei. Staining of γ-tubulin revealed that the dexrazoxane-treated cells contained multiple centrosomes, indicating that dexrazoxane prevents cytokinesis but not centrosome reduplication. It is concluded that dexrazoxane inhibits CHO cytokinesis in cells by virtue of its ability to inhibit topoisomerase II.
Footnotes
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Send reprint requests to: Dr. Brian B. Hasinoff, Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. E-mail: B_Hasinoff{at}UManitoba.CA
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↵1 This study was supported in part by the Medical Research Council of Canada (to B.B.H) and by National Institutes of Health National Cancer Institute Grants CA77468 and CA74972 (to J.C.Y.).
- Abbreviations:
- CHO
- Chinese hamster ovary
- FITC
- fluorescein isothiocyanate
- MTT
- 3-[4,5-dimethylthiazol-2-yl]-2,5-tetrazolium bromide
- PIPES
- piperazine-N,N′-bis(2-ethanesulfonic acid)
- Received May 16, 2000.
- Accepted July 20, 2000.
- U.S. Government
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