Abstract
Mercuric citrate with radiomercury was administered to anesthetized dogs and the excretion of radiomercury in urine was measured. Etimacrynic acid, given intravenously immediately after the injection of mercury produced a large diuretic response. It did not modify the urinary excretion of radiomercury. In contrast, ethacrynic acid did not produce a large diuretic response when the drug was administered two hours after the injaction of merucry. In this case the excretion of radiomercury decreased and this effect lasted for about 30 minutes. Other diuretics (furosemide, mannitol or hydroclorothiazide) did not alter the excretion of radiomercury, nor did mercury influence their diuretic activity. The nondiuretic compounds probenecid and an epoxide derivative of ethacrynic acid did not alter the excretion of radiomercury. Cysteine partially or completely restored the diuretic activity of ethacrynic acid and abolished the effects of ethacrynic acid on the excretion of radiomercury. These findings indicate that ethacrynic acid and mercury 1) compete for the same excretory carrier (probably a soluble thiol) and 2) compete for the same diuretic receptor (probably a protein-bound sulfhydryl).
Footnotes
- Received February 28, 1973.
- Accepted April 26, 1973.
- © 1973 by The Williams & Wilkins Co.
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