Abstract
Tritiated chloramphenicol which had entered E. coli cells was not associated with the soluble RNA fraction. A soluble fraction was prepared from 33,500 x g ribonucleoprotein particles which actively incorporated labeled amino acids in vitro. This fraction, and the residual 33,500 x g insoluble particle, exhibited a higher ratio of radioactivity to protein than the 105,000 x g supernatant fraction when prepared from cells exposed to tritiated chloramphenicol. However, an exchange reaction between H3-chloramphenicol and protein upon acid precipitation restricted interpretation of this observation.
Footnotes
- Received September 23, 1960.
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