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- Data Supplement - Supplemental Data. Confocol imaging of GluR1 and GluR2 signals under permeablizing or non-permeablizing conditions. Hippocampal slices were co-incubated with anti-N-terminal GluR1 and GluR2 antibodies. In the absence of detergent, surface GluR1 and GluR2 subunits were labeled (upper panel); while in the presence of detergent, surface and cytosolic GluR1 and GluR2 subunits were immunostained (lower panel). Scale bar: 20 µm.