Abstract
1. The cobalt-isopropylamine color reaction for the determination of amytal has been studied critically. Optimal amounts of reagents are recommended which reduce the method error to less than 2 per cent for concentrations of amytal of 20-300 mgm. per cent.
2. Dog urine normally contains pigments which are extracted by chloroform and which are included in the total color of the amytal reaction. This factor has been accounted for.
3. Dog urine normally also contains non-barbiturate chromogenic substances extracted by chloroform which produce color in the amytal reaction. An attempt has been made to estimate this factor quantitatively.
4. Dogs fed amytal excrete, in the urine, an increased amount of color producing substances. This increase cannot be taken as proof of amytal excretion.
Footnotes
- Received December 3, 1940.
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