Abstract
A method is described which allows the determination of ionized calcium in physiologic salt solutions.
The effect of bicarbonate and phosphate buffers on the ionization of calcium salts in Ringer solution has been studied.
Moderate concentrations of sodium bicarbonate (0.004 to 0.015 per cent) do not disionize calcium salts; higher concentrations (0.03 per cent) cause only a moderate disionization.
Phosphate buffers in concentrations of 0.008 to 0.048 per cent give rise to a marked reduction of the ionized calcium corresponding to their concentration.
This effect of phosphate buffers can be reduced to a great extent by the addition of 0.008 to 0.015 per cent of sodium bicarbonate.
These results parallel the effects of these buffers on the movements of the isolated intestine.
The method described is being adapted to the determination of ionized calcium in serum. This will be reported in a future publication.
Footnotes
- Received October 5, 1931.
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