Fig. 3. Pharmacological activation of TRPV3 by carvacrol inhibits hair growth. (A) Top panel, a schematic drawing for generation of TRPV3 agonist carvacrol-induced hair growth inhibition in C57BL/6 mice. Bottom panel, mice (7–8 weeks) were shaved and given different compounds topically once a day until hair grew out, images of hair growth were recorded every 3 days. Scale bar, 500 μm. (B) Summary from (A) for hair length randomly plucked and measured (n = 8) at different time intervals (9, 12, 15, 18, and 21 day) after topical application of different concentrations of TRPV3 agonist carvacrol (Car) and antagonist forsythoside B. Data are presented as the mean ± S.D. (****P < 0.0001, by two-way ANOVA, followed by Dunnett’s test.) (C) A self-comparison group between the left and right side of the skin after topical application of 191.1 mM carvacrol and vehicle (50% ethanol), respectively, once a day, and images for hair growth were recorded every 3 days. Scale bar, 500 μm. (D) Summary from (C) for comparison of hair length randomly plucked (n = 8) between left (L) and right (R) side of the skin. Data are presented as the mean ± S.D. (****P < 0.0001, by two-way ANOVA, followed by Sidak’s test.)