Abstract
As a result of the growing availability of genetically engineered mouse lines, the pilocarpine post–status epilepticus (SE) model of temporal lobe epilepsy is increasingly used in mice. A discrepancy in pilocarpine sensitivity in FVB/N wild-type versus P-glycoprotein (PGP)–deficient mice precipitated the investigation of the interaction between pilocarpine and two major multidrug transporters at the blood-brain barrier. Doses of pilocarpine necessary for SE induction were determined in male and female wild-type and PGP-deficient mice. Brain and plasma concentrations were measured following low (30–50 mg⋅kg−1 i.p.) and/or high (200 mg⋅kg−1 i.p.) doses of pilocarpine in wild-type mice, and mice lacking PGP, breast cancer resistance protein (BCRP), or both transporters, as well as in rats with or without pretreatment with lithium chloride or tariquidar. Concentration equilibrium transport assays (CETA) were performed using cells overexpressing murine PGP or BCRP. Lower pilocarpine doses were necessary for SE induction in PGP-deficient mice. Brain-plasma ratios were higher in mice lacking PGP or PGP and BCRP, which was also observed after pretreatment with tariquidar in mice and in rats. Lithium chloride did not change brain penetration of pilocarpine. CETA confirmed transport of pilocarpine by PGP and BCRP. Pilocarpine is a substrate of PGP and BCRP at the rodent blood-brain barrier, which restricts its convulsive action. Future studies to reveal whether strain differences in pilocarpine sensitivity derive from differences in multidrug transporter expression levels are warranted.
Footnotes
- Received December 11, 2014.
- Accepted March 6, 2015.
↵1 Current affiliation: Department of Nuclear Medicine, Preclinical Molecular Imaging, Hannover Medical School, Hannover, Germany.
K.R. and J.P.B. contributed equally to this work.
This work was supported by a grant from the German Research Foundation (DFG) [Grant Lo 274/10-2]. The authors have no conflicts of interest.
- Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics
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