The second messenger cAMP is involved in a number of cellular signaling pathways. In mammals, cAMP is produced by either the hormonally responsive, G protein-regulated transmembrane adenylyl cyclases (tmACs) or by the bicarbonate- and calcium-regulated soluble adenyl cyclases (sAC). To develop tools to differentiate the tmAC and sAC signaling, this study determined the specificity and potency of commercially available adenyl cyclase inhibitors. In cellular systems, two inhibitors, KH7 [2-(1H-benzo[d]imidazol-2-ylthio)-N′-(5-bromo-2-hydroxybenzylidene) propanehydrazide] and catechol estrogens proved to be specific for sAC, whereas 2′,5′-dideoxyadenosine proved to be specific for tmACs. These tools provide a means to define the specific contributions of the different families of adenylyl cyclases in cells and tissues, which will further our understanding of cell signaling.
See article at J Pharmacol Exp Ther 2013, 347:589–598.
- Copyright © 2013 by The American Society for Pharmacology and Experimental Therapeutics