Abstract
Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.
Footnotes
Article, publication date, and citation information can be found at http://jpet.aspetjournals.org.
doi:10.1124/jpet.110.172916.
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ABBREVIATIONS:
- SPECT
- single-photon emission computed tomography
- PET
- positron emission tomography
- FOV
- field of view
- Gy
- gray
- TE
- effective half-life
- TB
- biological half-life
- TI
- physical half-life of an isotope
- NHS
- N-hydroxy succinimidyl ester
- MAG3
- mercaptoacetyltriglycine
- DOTA
- 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid.
- Received July 29, 2010.
- Accepted October 14, 2010.
- Copyright © 2011 by The American Society for Pharmacology and Experimental Therapeutics
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