Abstract
Our research demonstrates that neonatal isolation (ISO; 1 h/day isolation; postnatal days 2–9) enhances extracellular, ventral striatal dopamine (DA) responses to psychostimulants in infant and juvenile rats. In adult rats, we find ISO facilitates acquisition and maintenance of cocaine self-administration. We now test whether ISO enhances cocaine-induced accumbens DA levels in adults using in vivo microdialysis. Behavioral responses to cocaine and DA antagonists were also examined. Adult male rats were derived from litters subjected to ISO or nonhandled (NH) control conditions. In experiment 1, microdialysis probes were aimed at accumbens core and separate groups administered vehicle or cocaine (5 and 10 mg/kg i.p.). Samples were analyzed for DA levels via high-performance liquid chromatography. In experiment 2, ISO and NH rats were administered one of these cocaine doses, and locomotor activity was assessed. Effects of cocaine (0.3–30 mg/kg), the D1 antagonist SCH23390 [R-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (0.003–0.03 mg/kg)], and the D2 antagonist eticlopride (0.01–0.1 mg/kg) on disruption of responding for food were examined in experiment 3. Cocaine plasma levels were assessed in experiment 4. ISO enhanced cocaine-induced increases in accumbens DA levels. Furthermore, the D2, but not D1, antagonist disrupted behavior to a greater extent in ISO versus NH rats. Yet, ISO did not significantly alter behavioral responses to cocaine or cocaine plasma levels. These data show that the ability of ISO to enhance accumbens DA responses to cocaine endures into adulthood. Moreover, that ISO rats are more sensitive to a D2 antagonist may reflect decreased levels of this receptor type as we showed previously in infant rats.
Footnotes
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This research was supported by the Patrick and Catherine Weldon Donaghue Foundation (DF 00-202), the VA New England Mental Illness Research Education and Clinical Center, and by the Yale International Women's Health Research Scholar Program on Women and Drug Abuse (1K12DA14038).
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doi:10.1124/jpet.104.081216.
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ABBREVIATIONS: DA, dopamine; NAc, nucleus accumbens; ISO, neonatal isolation; NH, nonhandled; PN, postnatal; SCH23390, R-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine; HPLC, high-performance liquid chromatography; FR, fixed ratio; ANOCOVA, analysis of covariance.
- Received November 22, 2004.
- Accepted April 19, 2005.
- The American Society for Pharmacology and Experimental Therapeutics
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