Abstract
Rho-kinase regulates the actin cytoskeleton and therefore modulates transport. The role of Rho-kinase in Na-H exchanger (NHE) activity of rat proximal convoluted tubules (PCTs) was investigated using (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide (Y-27632), a specific inhibitor of Rho-kinase. In spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats, apical and basolateral NHE activities were determined by measuring cell pH recovery following luminal NH
Footnotes
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DOI: 10.1124/jpet.102.041871
- Abbreviations:
- NHE
- sodium-hydrogen exchanger
- SHR
- spontaneously hypertensive rat
- Y-27632
- (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide
- WKY
- Wistar Kyoto
- BP
- blood pressure
- BCECF-AM
- acetoxymethyl ester of 2′,7′-bis(carboxyethyl) carboxyfluorescein
- FE
- fractional excretion
- GFR
- glomerular filtration rate
- BBMV
- brush-border membrane vesicle
- MES
- 4-morpholineethanesulfonic acid
- CHO
- Chinese hamster ovary
- Received July 18, 2002.
- Accepted October 22, 2002.
- The American Society for Pharmacology and Experimental Therapeutics
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