Abstract
Mesocorticolimbic dopamine (DA) transmission has been implicated in the consummatory and, more recently, the incentive-motivational aspect of ethanol's actions. The purpose of this study was to test whether ethanol-seeking behavior induced by an ethanol-associated contextual stimulus is sensitive to antagonism of DA transmission. Male Wistar rats were trained to orally self-administer 10% ethanol and to associate olfactory discriminative stimuli with the availability of ethanol (S+) versus nonreward (S−). Ethanol-reinforced operant responding then was extinguished by withholding ethanol and the associated S+. After reaching a predetermined extinction criterion, reinstatement tests were conducted in which the animals were presented noncontingently with only the S+ or S−. Exposure to the S+ but not the S− reinstated responding at the previously active lever. The D1 antagonistR(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride (SCH23390; 5, 10, 50 μg/kg s.c.) and the D2 antagonist eticlopride (5, 10, 50 μg/kg s.c.) dose dependently decreased the number of S+-induced responses and increased response latency. During a second test, conducted in the same rats, 3 weeks after withdrawal from a 12-day ethanol vapor inhalation procedure, the response-reinstating efficacy of the S+remained unaltered. However, the potency of both DA antagonists to inhibit the S+-induced drug-seeking response was significantly increased. The results confirm that ethanol-related contextual stimuli reliably elicit drug-seeking behavior and suggest that this effect requires activation of DA neurotransmission. The results also indicate that chronic ethanol exposure produces changes in D1 and D2 receptor function that lead to enhanced sensitivity to the behavioral effects of antagonists for these receptors.
Footnotes
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This work was supported by National Institutes of Health Grant AA10531 from the National Institute on Alcohol Abuse and Alcoholism (to F.W.). This is publication number 14287-NP from The Scripps Research Institute.
- Abbreviations:
- DA
- dopamine
- NAcc
- nucleus accumbens
- S+
- discriminative stimulus associated with ethanol reward
- S−
- discriminative stimulus associated with nonreward
- ANOVA
- analysis of variance
- SCH23390
- R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride
- Received October 4, 2001.
- Accepted November 28, 2001.
- The American Society for Pharmacology and Experimental Therapeutics
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