Abstract
ATP, coreleased with norepinephrine, affects adrenergic transmission by acting on purinoceptors at sympathetic nerve endings. Ectonucleotidases terminate the actions of ATP. Previously, we had preliminary evidence for ectonucleotidase activity in cardiac sympathetic nerve terminals. Therefore, we investigated whether this ectonucleotidase might influence norepinephrine release in the heart. Sympathetic nerve endings isolated from guinea pig heart (cardiac synaptosomes) were rich in Ca2+-dependent ectonucleotidase activity, as measured by metabolism of exogenously added radiolabeled ATP or ADP. By its inhibitor profile, ectonucleotidase resembled ectonucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1). Exogenous ATP elicited concentration-dependent norepinephrine release from cardiac synaptosomes (EC50 0.96 μM). This release was antagonized by the P2X receptor antagonist pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) (10 μM) and potentiated by the P2Y receptor antagonist 2′-deoxy-N6-methyladenosine-3′,5′-diphosphate (MRS 2179) (30 nM). Norepinephrine release promoted by ATP was also potentiated by the nucleotidase inhibitor 6-N,N-diethyl-β-γ-dibromomethylene-d-adenosine-5′-triphosphate (ARL67156) (30 μM) and blocked by a recombinant, soluble form of human E-NTPDase1 (solCD39). In contrast, ARL67156 had no effect on norepinephrine release induced by the nonhydrolyzable analog, α, β-methyleneadenosine-5′-triphosphate (α,β-MeATP). Depolarization of cardiac synaptosomes with K+ elicited release of endogenous norepinephrine. This was attenuated by PPADS and solCD39 and potentiated by MRS 2179 and ARL67156. Importantly, our results demonstrate that facilitation of ATP-induced norepinephrine release from cardiac sympathetic nerves is a composite of two autocrine components: positive, mediated by P2X receptors, and negative, mediated by P2Y receptors. Modulation of norepinephrine release by coreleased ATP is terminated by endogenous as well as exogenous ectonucleotidase. We propose that ectonucleotidase control of norepinephrine release should provide cardiac protection in hyperadrenergic states such as myocardial ischemia.
Footnotes
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Supported by National Institutes of Health Grants HL 34215 (R.L., C.S.), HL 46403 (A.J.M., M.J.B., R.L.), HL 47073, and NS 41462 (A.J.M., M.J.B.), and by Merit Review grants from the Department of Veterans Affairs (A.J.M., M.J.B., J.H.F.D.). A preliminary version of these findings was presented at Experimental Biology 2001 and was published in abstract form in FASEB J15:A552.
- Abbreviations:
- NE
- norepinephrine
- ARL67156
- 6-N,N-diethyl-β-γ-dibromomethylene-d-adenosine-5′-triphosphate
- DEPC
- diethylpyrocarbonate
- E-NTPDase1
- ectonucleoside triphosphate diphosphohydrolase 1
- HBS
- HEPES-buffered saline
- α
- β-MeATP, α, β-methyleneadenosine-5′-triphosphate
- 2-MeSATP
- 2-methylthioadenosine-5′-triphosphate
- MRS 2179
- 2′-deoxy-N6-methyladenosine-3′,5′-diphosphate
- PPADS
- pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid
- TLC
- thin layer chromatography
- Received September 28, 2001.
- Accepted November 7, 2001.
- The American Society for Pharmacology and Experimental Therapeutics
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