Differential Activation of Extracellular Signal Regulated Kinase Isoforms in Preconditioning and Opioid-Induced Cardioprotection
Abstract
Stimulation of the δ1-opioid receptor has been shown to trigger ischemic preconditioning (IPC). Additionally, myocardial ischemia/reperfusion induces the activation of extracellular signal-regulated kinase (ERK). Therefore, we examined the role of ERK in acute cardioprotection induced by δ1-opioid receptor stimulation or IPC. Infarct size (IS) was expressed as a percentage of the area at risk (AAR). Control animals had an IS/AAR of 60.6 ± 1.8. IPC and δ1-opioid receptor stimulation with TAN-67 reduced IS/AAR (8.2 ± 1.3 and 30.2 ± 2.4). Inhibition of ERK with the selective MEK-1 antagonist, PD 098059 during IPC or TAN-67 administration significantly reduced cardioprotection (41.5 ± 6.4 and 63.0 ± 4.8). Western Blot analysis and subsequent densitometry corroborated these observations. Control, TAN-67-, or IPC-treated hearts were harvested after 0, 5, 15, and 30 min of ischemia or 5, 30, and 60 min of reperfusion and separated into cytosolic and nuclear fractions. Both isoforms of ERK (p44 and p42) rapidly increased to greater levels throughout reperfusion in the nuclear fraction of IPC- and opioid-treated versus control rats, however, this increase was not attenuated by PD 098059. Conversely, the rapid activation of the 44-kDa isoform of ERK after 5 min of reperfusion in the cytosolic fraction was significantly increased in IPC- and opioid-treated hearts versus control, and this increase was abolished by pretreatment with PD 098059. Additionally, p42 was activated in the cytosolic fraction of IPC-treated animals. These results suggest a key role for the 44-kDa isoform of ERK in the cytoplasm during cardioprotection induced by either IPC or stimulation of the δ1-opioid receptor.
Footnotes
-
Send reprint requests to: Garrett J. Gross, Ph.D., Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226. E-mail:ggross{at}mcw.edu
-
This study was funded in part by a predoctoral research grant from the American Heart Association (R.M.F.) and National Institutes of Health Grant HL08311 (G.J.G.).
- Abbreviations:
- IPC
- ischemic preconditioning
- AAR
- area at risk
- ERK
- extracellular signal-regulated kinase
- HDAC-1
- histone deacetylase-1
- HR
- heart rate
- IS
- infarct size
- JNK
- c-jun N-terminal kinase
- KATP
- ATP-sensitive potassium channel
- LV
- left ventricular weight
- MAP
- mitogen-activated protein
- PKC
- protein kinase C
- TTC
- 2,3,5-triphenyltetrazolium chloride
- TAN-67
- 2-methyl-4aα-(3-hydroxyphenyl)-1,2,3,4,4a,5,12,12aα-octahydroquinolino[2,3,3-g]isoquinoline
- PD 098059
- 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
-
- Received August 17, 2000.
- Accepted October 12, 2000.
- The American Society for Pharmacology and Experimental Therapeutics
