Abstract
The effect of sympathectomy on parasympathetic regulation of ocular perfusion was investigated. Uveal blood flow through the vortex veins was measured by laser Doppler flowmetry during electrical stimulation of the superior salivatory nucleus, which activates ocular parasympathetic nerves, in adult rats with intact innervation and 2 days or 6 weeks after excision of the ipsilateral superior cervical ganglion. In all groups, parasympathetic stimulation produced comparable increases in flux, which were abolished by the selective neuronal nitric-oxide synthetase inhibitor, 1-(2-trifluoromethylphenyl) imidazole. Atropine had no effect in control and acutely sympathectomized rats but abolished the flux increase in four of six chronically sympathectomized animals, and 1-(2-trifluoromethylphenyl) imidazole eliminated the residual response. The muscarinic receptor agonist bethanechol did not affect basal flow in control or sympathectomized rats. However, bethanechol enhanced parasympathetically mediated vasodilation, but only in rats studied at 6 weeks after sympathectomy, a finding consistent with the appearance of muscarinic prejunctional facilitation of nitrergic transmission. In chronically sympathectomized rats, the M2 and M4 receptor antagonists methoctramine and tropicamide did not affect choroidal flow during parasympathetic activation. However, pirenzepine increased flux, implying the presence of M1inhibitory autoreceptors on these nerves. Parasympathetically mediated increased flux was partially blocked by the M3 antagonist 4-diphenylacetoxy-N-methylpiperdine, and the remaining vasodilation was blocked by atropine. We conclude that parasympathetic prejunctional facilitatory M3 and probably M5 receptors adopt a crucial role after chronic sympathectomy in maintaining nitrergic vasodilatory ocular neurotransmission in the face of down-regulated nitric oxide transmitter mechanisms.
Footnotes
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Send reprint requests to: Dr. Peter G. Smith, Dept. of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160-7410. E-mail: psmith{at}kumc.edu
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↵1 This work was supported by National Institutes of Health Grant HD33025, with core support from center grant HD02528 from the National Institute of Child Health and Human Development.
- Abbreviations:
- SSN
- superior salivatory nucleus
- TRIM
- 1-(2-trifluoromethylphenyl) imidazole
- 4-DAMP
- 4-diphenylacetoxy-N-methylpiperdine
- Received December 23, 1999.
- Accepted April 27, 2000.
- The American Society for Pharmacology and Experimental Therapeutics
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